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nf-core<p>Pipeline release! nf-core/mag v3.4.0 - mag 3.4.0 - Green Gecko!</p><p>Please see the changelog: <a href="https://github.com/nf-core/mag/releases/tag/3.4.0" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">github.com/nf-core/mag/release</span><span class="invisible">s/tag/3.4.0</span></a></p><p><a href="https://mstdn.science/tags/annotation" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>annotation</span></a> <a href="https://mstdn.science/tags/assembly" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>assembly</span></a> <a href="https://mstdn.science/tags/binning" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>binning</span></a> <a href="https://mstdn.science/tags/longreadsequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>longreadsequencing</span></a> <a href="https://mstdn.science/tags/metagenomes" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomes</span></a> <a href="https://mstdn.science/tags/metagenomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomics</span></a> <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/nanoporesequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanoporesequencing</span></a> <a href="https://mstdn.science/tags/nfcore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nfcore</span></a> <a href="https://mstdn.science/tags/openscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>openscience</span></a> <a href="https://mstdn.science/tags/nextflow" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nextflow</span></a> <a href="https://mstdn.science/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a></p>
Leszek<p>Super cool paper (part of PhD thesis): I thought assembling the genome from raw signal isn't practical, but it seems not possible, but yielding results comparable/better to usual approaches: basecalling followed by de novo genome assembly!<br><a href="https://genomic.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://genomic.social/tags/GenomeAssembly" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>GenomeAssembly</span></a><br><a href="https://arxiv.org/pdf/2410.17801" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="">arxiv.org/pdf/2410.17801</span><span class="invisible"></span></a><br><a href="https://arxiv.org/html/2503.02997v1#Ch7" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">arxiv.org/html/2503.02997v1#Ch</span><span class="invisible">7</span></a></p>
naturepoker<p>Looking at a funny discrepancy between Dorado 0.8.3 methylation-SUP basecalled bam vs 0.9.1 methylation-SUP basecalled bam. </p><p>They're the same raw files with same models, only Dorado version are different. </p><p>Left, 0.8.3, right, 0.9.1</p><p>That looks like one heck of a discrepancy for a few version numbers on basecalling software. </p><p><a href="https://genomic.social/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a> <a href="https://genomic.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a></p>
naturepoker<p>What do <a href="https://genomic.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://genomic.social/tags/sequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>sequencing</span></a> <a href="https://genomic.social/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a> folks think about this one? New preprint on hashing raw nanopore signals and using them directly for de-novo assembly. </p><p><a href="https://arxiv.org/abs/2503.02997v1" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="">arxiv.org/abs/2503.02997v1</span><span class="invisible"></span></a></p><p>Hypothetical blow5-to-assembly pipe would be interesting, though figuring it out is way out of my depth.</p>
Leszek<p>shamelessly sharing our recent work: Rapid and accurate <a href="https://genomic.social/tags/demultiplexing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>demultiplexing</span></a> of<br>direct <a href="https://genomic.social/tags/RNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>RNA</span></a> <a href="https://genomic.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> sequencing datasets with SeqTagger<br><a href="https://genomic.social/tags/paper" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>paper</span></a> <br><a href="https://genome.cshlp.org/content/early/2025/01/29/gr.279290.124.full.pdf" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">genome.cshlp.org/content/early</span><span class="invisible">/2025/01/29/gr.279290.124.full.pdf</span></a></p>
nf-core<p>Pipeline release! nf-core/taxprofiler v1.2.3 - v1.2.3 - Bouncy Basenji Patch!</p><p>Please see the changelog: <a href="https://github.com/nf-core/taxprofiler/releases/tag/1.2.3" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">github.com/nf-core/taxprofiler</span><span class="invisible">/releases/tag/1.2.3</span></a></p><p><a href="https://mstdn.science/tags/classification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>classification</span></a> <a href="https://mstdn.science/tags/illumina" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>illumina</span></a> <a href="https://mstdn.science/tags/longreads" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>longreads</span></a> <a href="https://mstdn.science/tags/metagenomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomics</span></a> <a href="https://mstdn.science/tags/microbiome" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microbiome</span></a> <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/pathogen" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>pathogen</span></a> <a href="https://mstdn.science/tags/profiling" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>profiling</span></a> <a href="https://mstdn.science/tags/shotgun" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>shotgun</span></a> <a href="https://mstdn.science/tags/taxonomicclassification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>taxonomicclassification</span></a> <a href="https://mstdn.science/tags/taxonomicprofiling" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>taxonomicprofiling</span></a> <a href="https://mstdn.science/tags/nfcore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nfcore</span></a> <a href="https://mstdn.science/tags/openscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>openscience</span></a> <a href="https://mstdn.science/tags/nextflow" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nextflow</span></a> <a href="https://mstdn.science/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a></p>
Una<p><a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/OxfordNanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>OxfordNanopore</span></a> <a href="https://mstdn.science/tags/NanoporeSequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>NanoporeSequencing</span></a> so just noticed that the 48 pack flowcells is no longer available from the Nanopore store. That is a big price hike for sequencing facilities who usually orders a big pack at a time. <span class="h-card" translate="no"><a href="https://genomic.social/@nanopore" class="u-url mention" rel="nofollow noopener noreferrer" target="_blank">@<span>nanopore</span></a></span></p>
Leszek<p>Shamelessly sharing our latest publication: De novo basecalling of <a href="https://genomic.social/tags/RNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>RNA</span></a> <a href="https://genomic.social/tags/modifications" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>modifications</span></a> at single molecule and nucleotide resolution<br><a href="https://genomic.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <br><a href="https://link.springer.com/epdf/10.1186/s13059-025-03498-6?sharing_token=IytKQ2p-kKbfSMJeCUsTt2_BpE1tBhCbnbw3BuzI2ROlz0B3e9LQUoHA9qh6NODgonYuX1G1GMfsxEe_sTuS_e_GRybSYX9mxrEoeASxC5RIlpmlV0mD6YesQ7BOjIbYdaxcUKYz3q8l2T2_bvSlO8Tykxj3ICWTyjPsKAmQ36s%3D" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">link.springer.com/epdf/10.1186</span><span class="invisible">/s13059-025-03498-6?sharing_token=IytKQ2p-kKbfSMJeCUsTt2_BpE1tBhCbnbw3BuzI2ROlz0B3e9LQUoHA9qh6NODgonYuX1G1GMfsxEe_sTuS_e_GRybSYX9mxrEoeASxC5RIlpmlV0mD6YesQ7BOjIbYdaxcUKYz3q8l2T2_bvSlO8Tykxj3ICWTyjPsKAmQ36s%3D</span></a></p>
nf-core<p>Pipeline release! nf-core/mag v3.3.1 - mag 3.3.1 - [2025-03-13]!</p><p>Please see the changelog: <a href="https://github.com/nf-core/mag/releases/tag/3.3.1" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">github.com/nf-core/mag/release</span><span class="invisible">s/tag/3.3.1</span></a></p><p><a href="https://mstdn.science/tags/annotation" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>annotation</span></a> <a href="https://mstdn.science/tags/assembly" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>assembly</span></a> <a href="https://mstdn.science/tags/binning" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>binning</span></a> <a href="https://mstdn.science/tags/longreadsequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>longreadsequencing</span></a> <a href="https://mstdn.science/tags/metagenomes" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomes</span></a> <a href="https://mstdn.science/tags/metagenomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomics</span></a> <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/nanoporesequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanoporesequencing</span></a> <a href="https://mstdn.science/tags/nfcore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nfcore</span></a> <a href="https://mstdn.science/tags/openscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>openscience</span></a> <a href="https://mstdn.science/tags/nextflow" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nextflow</span></a> <a href="https://mstdn.science/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a></p>
nf-core<p>Pipeline release! nf-core/taxprofiler v1.2.2 - v1.2.2 - Bouncy Basenji Patch!</p><p>Please see the changelog: <a href="https://github.com/nf-core/taxprofiler/releases/tag/1.2.2" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">github.com/nf-core/taxprofiler</span><span class="invisible">/releases/tag/1.2.2</span></a></p><p><a href="https://mstdn.science/tags/classification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>classification</span></a> <a href="https://mstdn.science/tags/illumina" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>illumina</span></a> <a href="https://mstdn.science/tags/longreads" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>longreads</span></a> <a href="https://mstdn.science/tags/metagenomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomics</span></a> <a href="https://mstdn.science/tags/microbiome" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microbiome</span></a> <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/pathogen" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>pathogen</span></a> <a href="https://mstdn.science/tags/profiling" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>profiling</span></a> <a href="https://mstdn.science/tags/shotgun" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>shotgun</span></a> <a href="https://mstdn.science/tags/taxonomicclassification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>taxonomicclassification</span></a> <a href="https://mstdn.science/tags/taxonomicprofiling" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>taxonomicprofiling</span></a> <a href="https://mstdn.science/tags/nfcore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nfcore</span></a> <a href="https://mstdn.science/tags/openscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>openscience</span></a> <a href="https://mstdn.science/tags/nextflow" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nextflow</span></a> <a href="https://mstdn.science/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a></p>
nf-core<p>Pipeline release! nf-core/mag v3.3.0 - mag 3.3.0 - Red Reindeer!</p><p>Please see the changelog: <a href="https://github.com/nf-core/mag/releases/tag/3.3.0" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">github.com/nf-core/mag/release</span><span class="invisible">s/tag/3.3.0</span></a></p><p><a href="https://mstdn.science/tags/annotation" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>annotation</span></a> <a href="https://mstdn.science/tags/assembly" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>assembly</span></a> <a href="https://mstdn.science/tags/binning" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>binning</span></a> <a href="https://mstdn.science/tags/longreadsequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>longreadsequencing</span></a> <a href="https://mstdn.science/tags/metagenomes" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomes</span></a> <a href="https://mstdn.science/tags/metagenomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomics</span></a> <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/nanoporesequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanoporesequencing</span></a> <a href="https://mstdn.science/tags/nfcore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nfcore</span></a> <a href="https://mstdn.science/tags/openscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>openscience</span></a> <a href="https://mstdn.science/tags/nextflow" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nextflow</span></a> <a href="https://mstdn.science/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a></p>
Christos Argyropoulos MD, PhD<p>Apparently, I am Mr. August for a patent of a protocol for <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> simultaneous sequencing of long and short RNAs <br><a href="https://www.biorxiv.org/content/10.1101/2022.12.16.520507v1.full" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/20</span><span class="invisible">22.12.16.520507v1.full</span></a><br><a href="https://mstdn.science/tags/UNM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>UNM</span></a></p>
Christos Argyropoulos MD PhD<p>Apparently, I am Mr. August for a patent of a protocol for <a href="https://mastodon.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> simultaneous sequencing of long and short RNAs <br><a href="https://www.biorxiv.org/content/10.1101/2022.12.16.520507v1.full" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/20</span><span class="invisible">22.12.16.520507v1.full</span></a><br><a href="https://mastodon.social/tags/UNM" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>UNM</span></a></p>
Abish Stephen<p>Today was a special day. <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/LongReadSequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>LongReadSequencing</span></a></p>
naturepoker<p>Very excited to dig into the paper - tool &amp; methodology the authors developed could save lots of headaches for my current sequencing project. </p><p>Can anyone reach the linked GitHub URL on the published article though? Entire NanoConsensus repo seems to 404 on my end.<br><a href="https://genomic.social/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a> <a href="https://genomic.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://genomic.social/tags/sequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>sequencing</span></a> </p><p>Native RNA nanopore sequencing reveals antibiotic-induced loss of rRNA modifications in the A- and P-sites | Nature Communications<br><a href="https://www.nature.com/articles/s41467-024-54368-x" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">nature.com/articles/s41467-024</span><span class="invisible">-54368-x</span></a></p>
nf-core<p>Pipeline release! nf-core/taxprofiler v1.2.1 - v1.2.1 - Bouncy Basenji Patch [2024-11-28]!</p><p>Please see the changelog: <a href="https://github.com/nf-core/taxprofiler/releases/tag/1.2.1" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">github.com/nf-core/taxprofiler</span><span class="invisible">/releases/tag/1.2.1</span></a></p><p><a href="https://mstdn.science/tags/classification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>classification</span></a> <a href="https://mstdn.science/tags/illumina" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>illumina</span></a> <a href="https://mstdn.science/tags/longreads" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>longreads</span></a> <a href="https://mstdn.science/tags/metagenomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomics</span></a> <a href="https://mstdn.science/tags/microbiome" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microbiome</span></a> <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/pathogen" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>pathogen</span></a> <a href="https://mstdn.science/tags/profiling" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>profiling</span></a> <a href="https://mstdn.science/tags/shotgun" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>shotgun</span></a> <a href="https://mstdn.science/tags/taxonomicclassification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>taxonomicclassification</span></a> <a href="https://mstdn.science/tags/taxonomicprofiling" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>taxonomicprofiling</span></a> <a href="https://mstdn.science/tags/nfcore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nfcore</span></a> <a href="https://mstdn.science/tags/openscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>openscience</span></a> <a href="https://mstdn.science/tags/nextflow" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nextflow</span></a> <a href="https://mstdn.science/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a></p>
naturepoker<p>Comparing what should be ONT r10 reads from two different third party sequencing providers.</p><p>Quality differences between the two are VERY noticeable, despite both companies likely using similar methods to keep the prices down. </p><p>I guess your choice of sequencing provider does matter! </p><p><a href="https://genomic.social/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a> <a href="https://genomic.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://genomic.social/tags/microbiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microbiology</span></a> <a href="https://genomic.social/tags/sequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>sequencing</span></a></p>
nf-core<p>Pipeline release! nf-core/detaxizer v1.1.0 - detaxizer 1.1.0 - Kombjuudr!</p><p>Please see the changelog: <a href="https://github.com/nf-core/detaxizer/releases/tag/1.1.0" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">github.com/nf-core/detaxizer/r</span><span class="invisible">eleases/tag/1.1.0</span></a></p><p><a href="https://mstdn.science/tags/deidentification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>deidentification</span></a> <a href="https://mstdn.science/tags/decontamination" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>decontamination</span></a> <a href="https://mstdn.science/tags/edna" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>edna</span></a> <a href="https://mstdn.science/tags/fastq" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>fastq</span></a> <a href="https://mstdn.science/tags/filter" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>filter</span></a> <a href="https://mstdn.science/tags/longreads" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>longreads</span></a> <a href="https://mstdn.science/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metabarcoding</span></a> <a href="https://mstdn.science/tags/metagenomics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>metagenomics</span></a> <a href="https://mstdn.science/tags/microbiome" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microbiome</span></a> <a href="https://mstdn.science/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <a href="https://mstdn.science/tags/shortreads" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>shortreads</span></a> <a href="https://mstdn.science/tags/shotgun" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>shotgun</span></a> <a href="https://mstdn.science/tags/taxonomicclassification" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>taxonomicclassification</span></a> <a href="https://mstdn.science/tags/taxonomicprofiling" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>taxonomicprofiling</span></a> <a href="https://mstdn.science/tags/nfcore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nfcore</span></a> <a href="https://mstdn.science/tags/openscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>openscience</span></a> <a href="https://mstdn.science/tags/nextflow" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nextflow</span></a> <a href="https://mstdn.science/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>bioinformatics</span></a></p>
Leszek<p>VERY excited to share SeqTagger with the world! <a href="https://github.com/novoalab/SeqTagger/" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://</span><span class="">github.com/novoalab/SeqTagger/</span><span class="invisible"></span></a></p><p>If you want to learn more about our super-fast and accurate <a href="https://genomic.social/tags/demultiplexing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>demultiplexing</span></a> tool for <a href="https://genomic.social/tags/direct" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>direct</span></a> <a href="https://genomic.social/tags/RNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>RNA</span></a> <a href="https://genomic.social/tags/sequencing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>sequencing</span></a> (DRS), check out the thread below. <a href="https://genomic.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>nanopore</span></a> <br><a href="https://www.biorxiv.org/content/10.1101/2024.10.29.620808v1" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/20</span><span class="invisible">24.10.29.620808v1</span></a></p><p>We started developing SeqTagger which follows a very straightforward logic: Let’s demultiplex by basecalling the <a href="https://genomic.social/tags/DNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>DNA</span></a> <a href="https://genomic.social/tags/barcode" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>barcode</span></a> sequence (on an RNA pore) and aligning it to a set of reference barcode sequences.</p>
Glyn Moody<p><a href="https://mastodon.social/tags/NHS" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>NHS</span></a> to launch 'real-time surveillance system' to prevent future pandemics - <a href="https://www.theregister.com/2024/11/08/nhs_realtime_surveillance_pandemic/" rel="nofollow noopener noreferrer" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">theregister.com/2024/11/08/nhs</span><span class="invisible">_realtime_surveillance_pandemic/</span></a> "<a href="https://mastodon.social/tags/Nanopore" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Nanopore</span></a> <a href="https://mastodon.social/tags/DNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>DNA</span></a> screening technology may identify novel pathogens, propose treatments in as little as six hours" could be good...</p>